Pipette tips can also be a source of leachables, which are trace amounts of chemicals derived from materials or process equipment that can contaminate samples. Heavy metals, UV stabilizers, antioxidants, pigments, release agents, biocides, and surfactants are examples of potential leachables. Leachables are not present in high-quality tips made from 100 percent virgin polypropylene in a high-quality production plant.
It is recommended to change the pipette tip after every sample to avoid the risk of contamination with another sample.
Always use a new tip for each different liquid. Use the correct pipette for the volume that is to be dispensed. Never use the 200-1000 µl pipette to dispense volumes below 200 µl.
A contaminated pipette or contaminated tips can cause contamination of samples. Prevention: Use sterilized tips or sterilized filter tips and if possible autoclave the pipette. Change the tip after pipetting of each sample.
If you keep using the same tip, you might contaminate other samples, as well as your reagents. In many laboratory workflows, you will need to change tips so often, that a micropipette has a specific ejector button for this.
Drain a pipette with tip against the inner wall of the receiving vessel. Never forcibly expel any hazardous material from a pipette. Carefully eject the disposable pipette tips to minimize aerosol formation.
Sample-to-sample contamination (or carry-over contamination) may be a result of aerosols or liquid residue being carried over from one sample to the next sample. This sometimes occurs when the same pipette tips are used several times. To prevent carry-over contamination: Always change the pipette tip after each sample.
Never use a micropipette without a tip. Always pipette gently, releasing the plunger button slowly. Letting the button pop up causes liquid to splash into the tip and can contaminate the plunger.
Washing and reusing tips is a proven safe practice that has been validated by smart, proactive labs committed to turning single-use plastic into a multi-use resource to reduce waste, cut costs, and boost efficiency. The safe practice of washing and reusing disposable pipette tips will NOT damage lab equipment.
Broken glass and pipette tips must be placed into a puncture proof container (i.e. a biohazard sharps container) that can withstand autoclave temperatures and has a built in sterilization indicator or autoclave tape attached.
You must change pipette tips between each serial dilution because trace amounts of solutions will stick to the side of the pipette tip, which can affect concentrations of dilutions.
All VistaLab Technologies sterile disposables (Tips, Reagent Reservoirs, Wobble-not serological pipets) are good for 3 years from the Date of Manufacture found on the Certificate of Analysis included in each case; as long as the packaging is unopened and uncompromised. Non-sterile tips do not expire.
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