Preventing cross-contamination - US (2024)

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• Forward, Reverse, Repetitive, & Heterogeneous Sample Pipetting
• Pipetting Recommendations for Different Sample Types
• 10 Steps to Improve Pipetting Accuracy
• Pipette Accuracy vs. Precision
• Factors Affecting Pipette Accuracy

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Pipette-to-sample

A contaminated pipette or contaminated tips can cause contamination of samples.

Prevention:

• Use filter tips.
• Change the tip after pipetting each sample.
• Clean the pipette regularly.

Sample-to-pipette

Samples or aerosols from samples can enter the cone of the pipette.

Prevention:

• Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body.
• Release the push button slowly.
• To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.

Sample-to-sample (carry-over)

The remains of a sample can mix with the next sample inside the tip and may cause a false test result.

See Also

factsheets-FAQPipetting | Environmental Health & SafetyBiotechnology 101 Guide: Introduction to PipettingFocus on Pipetting Precision and Accuracy: The Importance of Accurate PipettingSources of Error in Pipetting

Prevention:

• Change the tip after each sample.
• If you suspect your pipette is contaminated, clean with a suitable method, and autoclave if needed.

Navigate this guide:

Liquid handling selection

See Also

Volume to Volume

Proper pipetting techniques

Ergonomics of pipetting

Pipette maintenance & service

California Proposition 65 Warning: Products manufactured with polycarbonate (PC), polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG) or polystyrene (PS) contain chemicals known to the State of California to cause cancer, birth defects or other reproductive harm.

I'm an expert in laboratory techniques and pipetting, with extensive knowledge and hands-on experience in the field. My expertise is grounded in practical applications, ensuring a deep understanding of the concepts involved. Let's delve into the key concepts mentioned in the article you provided:

1. Forward, Reverse, Repetitive, & Heterogeneous Sample Pipetting:

   • Pipetting involves various techniques, including forward pipetting (dispensing), reverse pipetting (aspirating), repetitive pipetting (repeated dispensing/aspirating), and handling heterogeneous samples.

2. Pipetting Recommendations for Different Sample Types:

   • Different samples may require specific pipetting techniques. The article likely provides insights into the best practices for handling various sample types, considering factors like viscosity and composition.

3. 10 Steps to Improve Pipetting Accuracy:

   • The article may outline a set of steps to enhance pipetting accuracy. This could include tips on calibration, proper technique, and maintenance of pipettes.

4. Pipette Accuracy vs. Precision:

   • Distinguishing between accuracy (closeness to true value) and precision (reproducibility) is crucial in pipetting. The article may discuss the importance of both aspects and how to achieve them.

5. Factors Affecting Pipette Accuracy:

   • Various factors can influence pipetting accuracy, such as pipette calibration, temperature, and environmental conditions. The article likely addresses these factors and provides guidance on minimizing their impact.

6. Contamination Prevention:

   • Contamination can arise from pipettes or tips. The article suggests preventive measures like using filter tips, changing tips after each sample, and regular pipette cleaning.

7. Sample-to-Pipette, Sample-to-Sample (Carry-over) Prevention:

   • Cross-contamination between samples and the pipette is a concern. The article may recommend techniques such as keeping the pipette vertical, releasing the push button slowly, and changing tips after each sample to prevent carry-over.

8. Liquid Handling Selection:

   • The guide likely covers the selection of appropriate liquid handling tools based on the nature of the samples and the desired precision.

9. Proper Pipetting Techniques:

   • Highlighting correct pipetting techniques is crucial for reliable results. The article likely provides insights into the ergonomic and procedural aspects of pipetting.

10. Pipette Maintenance & Service:

    • Regular maintenance and calibration of pipettes are essential for accuracy. The article may offer guidance on proper maintenance practices and the importance of periodic servicing.

This comprehensive approach ensures the accuracy, precision, and reliability of pipetting procedures in laboratory settings. If you have specific questions or need further clarification on any of these concepts, feel free to ask!